Consulta: creatorFacets:"Sihler,William"
Registros recuperados: 3
Data/hora: 18/05/2024 17:44:35

Characterization of a Chrysodeixis includens nucleopolyhedrovirus Isolate from Brazilian Cerrado and Assessment of its Co-Infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus
Provedor de dados: Brazilian Archives of Biology and Technology
Autores: Sanches,Márcio Martinello; Sihler,William; Silva,Cláudia Efigênia Pereira; Guimarães,Giovana Curcio; Benito,Norton Polo; Sosa-Gómez,Daniel Ricardo; de Souza,Marlinda Lobo.
Abstract Chrysodeixis includens has become the major Lepidopteran pest of soybean crops, especially in the Brazilian Cerrado (savanna) region. A native isolate of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) from this region, Buritis, MG, was assessed for its biological and molecular features. In addition, in vitro co-infection with Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), another virus of an important soybean pest, was tested. The ChinNPV-Buritis isolate presented an average LC50 of 7,750 occlusion bodies (OBs)/ml of diet in C. includens larvae. Analysis of restriction endonuclease profiles of viral DNA revealed similarities with previously described ChinNPV isolates IE, IF, and IG from Brazil, although the presence of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AgMNPV; Baculovirus; ChinNPV; Soybean looper; Velvetbean caterpillar.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100218

In vitro infectivity of Spodoptera frugiperda multiple nucleopolyhedrovirus to different insect cell lines
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Sihler,William; Souza,Marlinda Lobo de; Valicente,Fernando Hercos; Falcão,Rosana; Sanches,Marcio Martinello.
Abstract: The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Baculovirus; Biological control; Cell culture; Fall armyworm; In vitro production; SfMNPV.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100001

Molecular biology of baculovirus and its use in biological control in Brazil
Provedor de dados: Pesquisa Agropecuária Brasileira
Autores: Castro,Maria Elita Batista de; Souza,Marlinda Lobo de; Sihler,William; Rodrigues,Júlio Carlyle Macedo; Ribeiro,Bergmann Morais.
Baculoviruses are insect viruses found mainly in Lepidoptera. The family Baculoviridae is taxonomically divided in two genera, Nucleopolyhedrovirus and Granulovirus, which differ by occlusion body morphology. NPVs (Nucleopolyhedroviruses) have polyhedrical inclusion bodies (PIBs) containing multiple viral particles, while GVs (Granuloviruses) appear to be generally single particles occluded in oval shaped occlusion bodies. During the life cycle, two different viral progenies are produced: BV (Budded Virus) and PDV (Polyhedra Derived Virus), which are essential for the infectious process and virus propagation in host cells. Baculoviruses are being used for pest control and they are especially safe due to their specificity and invertebrate-restricted host...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nucleopolyhedrovirus; AgNPV; Anticarsia gemmatalis; Baculovirus replication; Gene expression; Viral insecticide.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X1999001000001